首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠内脂素/内脏脂肪素(visfatin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NAMPT含量。NAMPT（尼克酰胺磷酸核糖转移酶）是哺乳动物细胞中烟酰胺腺嘌呤二核苷酸（NAD）补救合成途径的关键酶，参与能量代谢、抗氧化反应等。研究发现，NAMPT与肿瘤发生发展密切相关，抑制NAMPT可诱导细胞凋亡、自噬，抑制细胞增殖和血管生成，具有抗肿瘤作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于体外实验中评估大鼠模型或离体样本的内脂素表达水平，可应用于肥胖相关代谢机制研究、脂肪组织功能分析、药物干预效果评价等科研领域。其兼容多种生物样本的特性支持研究者同时分析循环系统（血清/血浆）与局部组织（如脂肪、肝脏等）的内脂素分布特征，为探索其在能量代谢、炎症调控等生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nampt ELISA Kit; Pbef1 ELISA Kit; Nicotinamide phosphoribosyltransferase ELISA Kit; NAmPRTase ELISA Kit; Nampt ELISA Kit; EC 2.4.2.12 ELISA Kit; Pre-B-cell colony-enhancing factor 1 homolog ELISA Kit; PBEF ELISA Kit; Visfatin ELISA Kit
缩写：NAMPT
Uniprot No.：Q80Z29
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat visfatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-97 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 96-108 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of rat visfatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 98 | 94-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2001.7431.8671.8051.7071001.3041.1991.2521.154500.8350.8380.8370.739250.5340.5470.5410.44312.50.3480.3360.3420.2446.250.2770.2680.2730.1753.120.1710.1770.1740.07600.0980.0970.098 | ||||||
货期：3-5 working days
×
