首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠核因子κB(NF-κB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的NFKB1含量。NFKB1是一种转录因子，参与调节多种生物过程中的转录，与RELA/p65、RELB和NFKB2/p52形成复合物。其研究机制涉及基因表达变化，与应激和免疫信号通路激活相关，突变可能导致免疫缺陷。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外实验模型中炎症信号通路机制研究、药物作用靶点筛选或免疫调控实验的定量分析；适用于细胞刺激实验、动物模型血清学分析及分子机制研究等科研场景，为生命科学领域提供稳定可靠的NF - κB蛋白定量解决方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nfkb1 ELISA Kit; Nuclear factor NF-kappa-B p105 subunit ELISA Kit; DNA-binding factor KBF1 ELISA Kit; EBP-1 ELISA Kit; NF-kappa-B1 p84/NF-kappa-B1 p98 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1) [Cleaved into: Nuclear factor NF-kappa-B p50 subunit] ELISA Kit
缩写：NFKB1
Uniprot No.：P25799
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of mouse NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.1762.2372.2072.004101.6351.6421.6391.43651.1841.2011.1930.9902.50.7950.8130.8040.6011.250.6010.6370.6190.4160.6250.4350.4460.4410.2380.3120.3330.3410.3370.13400.2010.2040.203 | ||||||
货期：3-5 working days
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