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产品描述：猪核因子-κB(NF-κB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NF-κB含量。NF - κB是重要的转录因子，广泛存在于多种细胞中。在细胞的存活、增殖、炎症反应等生理病理过程中起到关键作用。研究机制上，它受多种信号通路调控，激活后能转位至细胞核调控基因表达，其异常与肿瘤、炎症等多种疾病相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于动物模型构建、病原体感染机制研究、疫苗效力评估等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NF-kappa B,Nuclear factor-kappa B
缩写：NF-κB
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-106 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 88-95 | |||||
回收率：
| The recovery of pig NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 90 | 86-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2012.1012.1512.04710001.8241.7611.7931.6895001.4651.5301.4981.3942501.2101.1561.1831.0791250.7450.7990.7720.66862.50.3560.3410.3490.24531.250.2480.2570.2530.14900.1020.1050.104 | ||||||
货期：3-5 working days
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