| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP1B2 |
| Uniprot No | P14415 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 68-290aa |
| 氨基酸序列 | DHTPKYQDRL ATPGLMIRPK TENLDVIVNV SDTESWDQHV QKLNKFLEPY NDSIQAQKND VCRPGRYYEQ PDNGVLNYPK RACQFNRTQL GNCSGIGDST HYGYSTGQPC VFIKMNRVIN FYAGANQSMN VTCAGKRDED AENLGNFVMF PANGNIDLMY FPYYGKKFHV NYTQPLVAVK FLNVTPNVEV NVECRINAAN IATDDERDKF AGRVAFKLRI NKT |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP1B2重组蛋白的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *Expression and functional characterization of recombinant ATP1B2 in mammalian cells*
**作者**: Smith A, et al.
**摘要**: 本研究成功在HEK293细胞中表达了重组ATP1B2蛋白，并验证其与钠钾泵α亚基的相互作用，证实其在维持细胞膜离子梯度中的关键作用。
2. **文献名称**: *ATP1B2 recombinant protein modulates astrocyte migration in vitro*
**作者**: Chen L, et al.
**摘要**: 通过大肠杆菌表达系统纯化ATP1B2重组蛋白，发现其能够通过调节ERK信号通路影响星形胶质细胞的迁移能力，提示其在神经系统修复中的潜在应用。
3. **文献名称**: *Structural analysis of ATP1B2 using cryo-EM reveals ion transport mechanisms*
**作者**: Tanaka K, et al.
**摘要**: 利用冷冻电镜解析重组ATP1B2与α亚基复合物的高分辨率结构，揭示了β2亚基在稳定钠钾泵构象及离子选择性中的分子机制。
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建议通过 **PubMed** 或 **Google Scholar** 检索关键词 "ATP1B2 recombinant" 或 "ATP1B2 expression" 获取最新文献。如需具体文献，请提供更详细的研究方向(如疾病模型、表达系统等)。
**Background of ATP1B2 Recombinant Protein**
ATP1B2 (ATPase Na+/K+ Transporting Subunit Beta 2) is a critical component of the Na+/K+-ATPase enzyme complex, which maintains electrochemical gradients across cell membranes by transporting sodium and potassium ions. This transmembrane protein belongs to the β-subunit family (β1. β2. β3. β4) that regulates the stability, trafficking, and functional activity of the catalytic α-subunit. ATP1B2 is predominantly expressed in neural tissues, the inner ear, and the kidney, playing roles in neuronal excitability, hearing, and ion homeostasis.
Recombinant ATP1B2 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells*) to produce purified, functional forms for research. Its production enables studies on Na+/K+-ATPase assembly, ion transport mechanisms, and interactions with partner proteins or ligands. Researchers utilize it to investigate diseases linked to ATP1B2 dysfunction, such as neurological disorders, hearing loss, or hypertension. Additionally, it serves as an antigen for antibody development or diagnostic tools.
Structural and functional analyses of ATP1B2 have revealed its role in cell adhesion and signaling beyond ion transport, implicating it in cancer metastasis and neurodegenerative conditions. Mutations or altered expression of ATP1B2 are associated with pathologies, making it a target for therapeutic exploration. The recombinant protein’s availability accelerates mechanistic insights and drug screening, bridging molecular biology with clinical applications.
(Word count: ~200)
*Note: Adjusted to meet the 400-word limit while retaining key points.
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