首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠N-Myc互作蛋白(NMI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NMI含量。小鼠N-Myc互作蛋白(NMI)是一种与NMYC和CMYC相互作用的蛋白质，参与细胞周期、凋亡和转化。NMI通过增强STAT介导的转录，在细胞因子IL2和IFN-γ的刺激下发挥作用，并在多种癌症中表达上调，可能成为癌症治疗的新靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于肿瘤生物学、免疫调控等基础研究领域，可应用于小鼠模型中的肿瘤微环境分析、免疫细胞功能研究或药物干预后NMI表达动态监测，为探索NMI在肿瘤抑制、炎症反应及细胞信号转导中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NmiN-myc-interactor ELISA Kit; Nmi ELISA Kit; N-myc and STAT interactor ELISA Kit
缩写：NMI
Uniprot No.：O35309
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NMI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 88-102 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-97 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 85-99 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 91-105 | ||||||
回收率：
| The recovery of mouse NMI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 90-98 | |||||
| EDTA plasma (n=4) | 99 | 91-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.6112.5692.5902.4705002.3922.3142.3532.2332501.9791.8981.9391.8191251.1301.1051.1180.99862.50.6450.6220.6340.51431.20.4250.4340.4300.31015.60.3150.3070.3110.19100.1220.1180.120 | |||||||
货期：3-5 working days
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