首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经调节肽U(NMU)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NMU含量。NMU即神经调节素U，是一种神经肽。它在能量代谢、心血管功能、生殖等方面发挥重要生理作用。研究机制主要是通过与受体结合，激活下游信号通路来调节多种生理过程，目前其在疾病治疗如肥胖症等方面的研究颇具潜力。试剂盒检测范围为0.9 ng/mL-60 ng/mL，特别适用于探究NMU在肥胖机制、神经信号传导或能量代谢相关疾病模型中的作用机制研究；为神经科学、代谢调控及生理病理学领域的科研工作者提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NmuNeuromedin-U [Cleaved into: Neuromedin precursor-related peptide 36 ELISA Kit; NURP36); Neuromedin precursor-related peptide 33 ELISA Kit; NURP33); Neuromedin-U-23 ELISA Kit; NmU-23)] ELISA Kit
缩写：NMU
Uniprot No.：Q9QXK8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NMU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-105 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-106 | |||||
回收率：
| The recovery of mouse NMU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.6092.5632.5862.462301.8421.8051.8241.700151.2511.3021.2771.1537.50.7650.8040.7850.6613.750.5130.5370.5250.4011.80.3040.3210.3130.1890.90.2370.2310.2340.11000.1260.1210.124 | ||||||
货期：3-5 working days
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