首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Nodal同源物(NODAL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NODAL含量。NODAL属转化生长因子-β超家族成员，在胚胎发育中对细胞命运决定、组织分化和器官形成至关重要。研究机制方面，它主要通过结合受体激活下游信号通路，调控基因表达。异常激活与肿瘤发生、转移等相关，是潜在抗癌靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于发育生物学研究、肿瘤微环境分析、干细胞分化机制探索等科研领域，特别针对需要评估NODAL在胚胎发育异常或肿瘤进展中功能的研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HTX5 ELISA Kit; MGC138230 ELISA Kit; Nodal ELISA Kit; Nodal Growth Differentiation Factor ELISA Kit; nodal homolog (mouse) ELISA Kit; Nodal homolog ELISA Kit; Nodal; Mouse; Homolog ELISA Kit; NODAL_HUMAN ELISA Kit
缩写：NODAL
Uniprot No.：Q96S42
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NODAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 97-108 | |||||
回收率：
| The recovery of human NODAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-102 | ||||
| EDTA plasma (n=4) | 103 | 96-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.6882.8802.7842.67825002.3412.1812.2612.15512501.5321.6381.5851.4796250.9570.9930.9750.8693120.5230.5310.5270.4211560.3700.3840.3770.271780.2420.2540.2480.14200.1050.1070.106 | ||||||
货期：3-5 working days
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