Recombinant Human TGIF2LX protein

TGIF2LX (TGFB Induced Factor Homeobox 2 Like X-linked) is a member of the TGIF homeodomain protein family, which plays roles in transcriptional regulation and developmental processes. It shares structural homology with TGIF2. featuring a conserved N-terminal homeodomain for DNA binding and a C-terminal repression domain involved in protein interactions. TGIF2LX is thought to arise from retrotransposition or duplication of the TGIF2 gene, leading to its X-chromosome localization. While its exact physiological functions remain under investigation, studies suggest it acts as a transcriptional repressor, modulating signaling pathways like TGF-β, BMP, and Wnt, which are critical in embryogenesis, cell differentiation, and tissue homeostasis.

Notably, TGIF2LX exhibits testis-specific expression patterns, implicating potential roles in germ cell development or reproductive biology. Dysregulation of TGIF2LX has been linked to pathological conditions, particularly germ cell tumors (e.g., testicular seminomas) and prostate cancer, where it may contribute to oncogenic processes through epigenetic alterations or aberrant transcriptional control. Its recombinant protein form is engineered for in vitro and in vivo studies to dissect molecular interactions, map binding partners, and explore therapeutic targeting. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant protein often includes affinity tags (e.g., His-tag) for purification and detection. Current research focuses on clarifying its role in disease mechanisms and evaluating its utility as a biomarker or intervention target.