| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFF1 |
| Uniprot No | P04155 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-84aa |
| 氨基酸序列 | EAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF |
| 预测分子量 | 33.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于TFF1重组蛋白的参考文献示例(注：文献为虚构示例，实际文献需通过学术数据库检索验证)：
1. **文献名称**: "Expression and purification of recombinant human TFF1 in Escherichia coli for functional studies"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化重组人TFF1蛋白，通过体外实验验证其促进胃黏膜细胞迁移的能力，为TFF1的分子机制研究提供工具。
2. **文献名称**: "Recombinant TFF1 inhibits apoptosis in gastric cancer cells via EGFR signaling pathway"
**作者**: Wang Y, et al.
**摘要**: 发现重组TFF1蛋白通过激活EGFR信号通路抑制胃癌细胞凋亡，提示其在肿瘤微环境中的潜在促癌作用。
3. **文献名称**: "TFF1 as a biomarker: Development of a recombinant protein-based ELISA for early gastric disease detection"
**作者**: Kim S, et al.
**摘要**: 利用重组TFF1蛋白开发高灵敏度ELISA检测方法，证实其在胃癌和胃炎患者血清中表达显著升高，具有临床诊断潜力。
4. **文献名称**: "Structural and functional characterization of recombinant TFF1 in mucosal healing"
**作者**: Müller C, et al.
**摘要**: 通过晶体结构解析和动物模型实验，阐明重组TFF1通过稳定黏液层和调节炎症因子加速肠道黏膜修复的分子机制。
建议通过PubMed、Web of Science等平台以“recombinant TFF1 protein”为关键词检索真实文献。
Trefoil Factor Family 1 (TFF1) is a small secreted protein belonging to the trefoil factor family, characterized by a conserved three-loop "trefoil" structural domain stabilized by disulfide bonds. Primarily expressed in mucosal tissues, particularly in the gastrointestinal tract, TFF1 plays a critical role in maintaining epithelial integrity, promoting mucosal repair, and regulating inflammatory responses. It is secreted by gastric epithelial cells and functions as a protective agent against physical and chemical damage, such as acid erosion or infection. TFF1 binds to mucins and cell surface receptors, enhancing mucus viscosity and stimulating cell migration to accelerate wound healing.
Recombinant TFF1 protein is produced using biotechnological platforms like *E. coli* or mammalian expression systems, enabling scalable purification for research and therapeutic applications. Its recombinant form retains the native protein’s bioactivity, facilitating studies on mucosal homeostasis, cancer biology (TFF1 is downregulated in gastric cancers), and inflammatory diseases. Researchers utilize recombinant TFF1 to explore its dual role in tumor suppression and potential oncogenic signaling in specific contexts. Clinically, it holds promise for treating mucosal injuries, peptic ulcers, or inflammatory bowel disease. Recent studies also investigate its interaction with microbial pathogens and immune modulation, expanding its relevance in infection and immunotherapy research. The development of TFF1-based biologics underscores its therapeutic potential in regenerative medicine.
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