| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFB1M |
| Uniprot No | Q8WVM0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-346aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMQAAKQLSQNFLLDLRLTDKIVRKAGNLTN AYVYEVGPGPGGITRSIL NADVAELLVVEKDTRFIPGLQMLSDAAPGK LRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTP LII KWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQY LCNVRHIFTIPGQAFVPKPEVD VGVVHFTPLIQPKIEQPFKLVEKVVQ NVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISH FKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TFB1M重组蛋白的3篇代表性文献的简要概括(注：文献信息为模拟示例，实际引用请核实真实来源)：
---
1. **标题**：*Recombinant Human TFB1M Expression in Escherichia coli and Its Role in Mitochondrial Transcription*
**作者**：Smith A, et al.
**摘要**：本研究成功构建了人源TFB1M重组蛋白的原核表达系统，通过优化诱导条件获得可溶性蛋白，并证实其与线粒体RNA聚合酶(POLRMT)的相互作用，揭示了TFB1M在调控线粒体DNA转录中的关键功能。
2. **标题**：*Structural Analysis of TFB1M Reveals Insights into Mitochondrial Ribosome Biogenesis*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了重组TFB1M蛋白的三维结构，发现其N端结构域与细菌转录因子具有同源性，提出了TFB1M在线粒体核糖体组装和代谢疾病(如2型糖尿病)中的潜在作用机制。
3. **标题**：*TFB1M Deficiency Impairs Insulin Secretion via Mitochondrial Dysfunction in Pancreatic β-Cells*
**作者**：Yamamoto K, et al.
**摘要**：利用重组TFB1M蛋白进行体外功能实验，证明其缺失导致β细胞线粒体转录活性下降、ATP合成减少，进而影响胰岛素分泌，为TFB1M与糖尿病的关联提供了分子证据。
---
**提示**：实际研究中，建议通过PubMed或Web of Science检索关键词“TFB1M recombinant protein”或“TFB1M mitochondrial”获取最新文献，重点关注蛋白功能、疾病模型及结构生物学研究。
**Background of TFB1M Recombinant Protein**
TFB1M (Transcription Factor B1 Mitochondrial), also known as methyltransferase-like protein 23 (METTL23), is a mitochondrial protein involved in regulating mitochondrial gene expression and energy metabolism. It plays a critical role in the assembly and stability of the mitochondrial transcription machinery, interacting with mitochondrial RNA polymerase (POLRMT) and transcription factor A (TFAM) to facilitate the transcription of mitochondrial DNA (mtDNA). TFB1M is also implicated in the methylation of mitochondrial ribosomal RNA, contributing to the proper functioning of mitochondrial translation.
Dysregulation of TFB1M has been linked to mitochondrial dysfunction, which is associated with metabolic disorders such as type 2 diabetes, neurodegenerative diseases, and aging. Studies suggest that TFB1M deficiency may impair mitochondrial biogenesis, reduce ATP production, and disrupt cellular energy homeostasis, highlighting its importance in metabolic regulation.
The recombinant TFB1M protein is produced using biotechnological methods, typically through expression in *E. coli* or mammalian cell systems. This involves cloning the TFB1M gene into an expression vector, followed by purification via affinity chromatography. Recombinant TFB1M retains its native structural and functional properties, making it a valuable tool for *in vitro* studies, such as investigating mitochondrial transcription mechanisms, protein-protein interactions, or screening potential therapeutic compounds targeting mitochondrial disorders.
Its applications extend to biomedical research, including elucidating disease pathways linked to mitochondrial dysfunction and developing diagnostic or therapeutic strategies. The availability of recombinant TFB1M has significantly advanced studies on mitochondrial biology and its role in human health and disease.
×
