| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TDP1 |
| Uniprot No | Q9NUW8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-608aa |
| 氨基酸序列 | MSQEGDYGRWTISSSDESEEEKPKPDKPSTSSLLCARQGAANEPRYTCSE AQKAAHKRKISPVKFSNTDSVLPPKRQKSGSQEDLGWCLSSSDDELQPEM PQKQAEKVVIKKEKDISAPNDGTAQRTENHGAPACHRLKEEEDEYETSGE GQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSA QFNYCFDVDWLVKQYPPEFRKKPILLVHGDKREAKAHLHAQAKPYENISL CQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIHADWHQKTQGIWLSP LYPRIADGTHKSGESPTHFKADLISYLMAYNAPSLKEWIDVIHKHDLSET NVYLIGSTPGRFQGSQKDNWGHFRLKKLLKDHASSMPNAESWPVVGQFSS VGSLGADESKWLCSEFKESMLTLGKESKTPGKSSVPLYLIYPSVENVRTS LEGYPAGGSLPYSIQTAEKQNWLHSYFHKWSAETSGRSNAMPHIKTYMRP SPDFSKIAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFGL DSFKVKQKFFAGSQEPMATFPVPYDLPPELYGSKDRPWIWNIPYVKAPDT HGNMWVPS |
| 预测分子量 | 93 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TDP1重组蛋白的参考文献示例(文献标题与作者为模拟信息，建议通过学术数据库验证)：
---
1. **标题**: *Expression and Functional Characterization of Recombinant Human Tyrosyl-DNA Phosphodiesterase 1 (TDP1)*
**作者**: Smith A, Jones B, et al.
**摘要**: 本研究报道了人源TDP1在大肠杆菌中的重组表达及纯化方法，通过亲和层析和离子交换技术获得高纯度蛋白，并验证其酶活性能有效修复Top1诱导的DNA损伤。
2. **标题**: *Structural Insights into TDP1 Catalytic Mechanism via Recombinant Protein Crystallography*
**作者**: Chen X, Wang Y, et al.
**摘要**: 利用重组表达的TDP1蛋白进行晶体结构解析，揭示了其催化活性位点的关键氨基酸残基，为设计靶向TDP1的抗癌药物提供结构基础。
3. **标题**: *Optimization of TDP1 Recombinant Production in Insect Cells for High-Throughput Drug Screening*
**作者**: Lee C, Kim D, et al.
**摘要**: 开发了一种在昆虫细胞中高效表达TDP1重组蛋白的体系，结合荧光底物建立高通量筛选平台，用于评估TDP1抑制剂在化疗增敏中的应用潜力。
4. **标题**: *Role of Recombinant TDP1 in Repairing Oxidative DNA Damage in Neuronal Cells*
**作者**: Patel R, Green M, et al.
**摘要**: 通过体外实验证实重组TDP1蛋白可修复神经元细胞中氧化应激导致的DNA单链断裂，提示其在神经退行性疾病治疗中的潜在价值。
---
**建议**：实际引用时，请通过PubMed或Google Scholar搜索关键词“recombinant TDP1 protein”或“TDP1 expression”，筛选近年或高被引文献。经典文献可能包括：
- Interthal, H. et al. (2001) *Nucleic Acids Res.* 对TDP1的克隆与初步表征。
- Pommier, Y. et al. (2006) *Nat. Rev. Cancer* 综述中提及TDP1的生物学功能及重组技术应用。
确保核对文献的完整信息及适用性。
TDP1 (Tyrosyl-DNA phosphodiesterase 1) is a DNA repair enzyme critical for resolving covalent DNA-protein crosslinks, particularly those involving topoisomerase 1 (TOP1). During DNA replication or transcription, TOP1 transiently forms a phosphotyrosyl bond with DNA to relieve torsional stress. When this process is disrupted by DNA damage or chemotherapeutic agents like camptothecin, TOP1 becomes trapped, generating cytotoxic TOP1-DNA adducts. TDP1 catalyzes the hydrolysis of these adducts, enabling DNA repair and genome stability.
Mutations in the TDP1 gene are linked to neurodegenerative disorders such as spinocerebellar ataxia with axonal neuropathy (SCAN1), highlighting its role in neuronal health. Additionally, TDP1 overexpression has been observed in certain cancers, where it may contribute to resistance against TOP1-targeting chemotherapy.
Recombinant TDP1 protein is produced using expression systems (e.g., E. coli or mammalian cells) for biochemical and therapeutic studies. Its applications include elucidating DNA repair mechanisms, screening TDP1 inhibitors to overcome drug resistance, and modeling disease-associated mutations. Structural studies of recombinant TDP1 have revealed its two-domain architecture and catalytic mechanism involving a conserved histidine-lysine-asparagine (HKN) motif.
Research on TDP1 recombinant protein bridges basic science and translational medicine, offering insights into neuroprotection strategies and combination therapies targeting DNA repair pathways in cancer.
×
