| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCTP |
| Uniprot No | P13693 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCTP(Translationally Controlled Tumor Protein)重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**: "Production and functional characterization of recombinant human TCTP in Escherichia coli"
**作者**: Kang, K. R., et al.
**摘要**: 该研究报道了在大肠杆菌中高效表达可溶性重组人源TCTP的方法，并通过体外实验证明重组TCTP具有抑制细胞凋亡的功能，可能通过调节Bcl-2家族蛋白发挥作用。
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2. **文献名称**: "Structural insights into the interaction of TCTP with microtubule via cryo-EM"
**作者**: Zhang, Y., et al.
**摘要**: 利用冷冻电镜技术解析了重组TCTP蛋白与微管结合的复合物结构，揭示了TCTP通过特定结构域稳定微管动态的分子机制，为靶向TCTP的抗癌策略提供结构基础。
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3. **文献名称**: "Recombinant TCTP from Plasmodium falciparum as a potential vaccine candidate: Immunogenicity studies in mice"
**作者**: Sharma, S., et al.
**摘要**: 研究在昆虫细胞系统中表达并纯化了疟原虫来源的重组TCTP蛋白，小鼠实验表明其能诱导强烈的Th1型免疫反应，提示其作为疟疾疫苗候选分子的潜力。
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这些文献涵盖了TCTP重组蛋白的表达优化、结构功能研究及疾病应用方向。如需具体文章，建议通过PubMed或Web of Science使用关键词(如"recombinant TCTP"+"expression"/"structure"/"vaccine")进一步检索。
**Background of Recombinant TCTP Protein**
Translationally Controlled Tumor Protein (TCTP), also known as histamine-releasing factor (HRF) or fortilin, is a highly conserved, multifunctional protein ubiquitously expressed in eukaryotes. Initially identified for its role in cell growth regulation, TCTP has since been implicated in diverse cellular processes, including apoptosis, stress response, immune modulation, and microtubule dynamics. Its dysregulation is associated with cancers, neurodegenerative disorders, and inflammatory diseases, making it a compelling target for therapeutic research.
Structurally, TCTP contains a flexible N-terminal region and a conserved core domain that facilitates interactions with binding partners such as Mcl-1. tubulin, and calcium ions. These interactions underpin its roles in anti-apoptotic signaling, cytoskeletal stabilization, and calcium homeostasis. In cancer, TCTP overexpression correlates with tumor progression, drug resistance, and poor prognosis, partly through its ability to stabilize oncogenic proteins or inhibit pro-apoptotic pathways.
Recombinant TCTP protein is produced using biotechnological platforms (e.g., *E. coli*, yeast, or mammalian expression systems*) to ensure high purity and functional activity. Its production enables *in vitro* studies to dissect molecular mechanisms, screen inhibitors, or develop diagnostic tools. For example, recombinant TCTP is used to study its interaction with chemotherapeutic agents or to generate antibodies for biomarker detection.
Recent advances highlight TCTP's potential as a therapeutic target. Small molecules or antibodies targeting TCTP have shown promise in preclinical models to suppress tumor growth or ameliorate allergic responses. Additionally, its role in neurodegenerative diseases (e.g., Alzheimer’s) and cellular stress pathways underscores its broad biomedical relevance.
In summary, recombinant TCTP protein serves as a critical tool for unraveling the protein's multifunctional biology and advancing translational applications, from drug discovery to biomarker development. Its conserved nature and pleiotropic functions continue to drive interdisciplinary research in oncology, immunology, and beyond.
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