| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFB2M |
| Uniprot No | Q9H5Q4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-396aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAGRFCILGSEAATRKHLPARNHCGLS DSSPQLWPEPDFRNPPRKASKASLDFKRYVTDRRLAETLAQIYLGKPSRP PHLLLECNPGPGILTQALLEAGAKVVALESDKTFIPHLESLGKNLDGKLR VIHCDFFKLDPRSGGVIKPPAMSSRGLFKNLGIEAVPWTADIPLKVVGMF PSRGEKRALWKLAYDLYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPD LYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQK LYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPL DARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLED R |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TFB2M重组蛋白的3篇参考文献概览：
1. **文献名称**：*"Reconstitution of Human Mitochondrial Transcription System with Recombinant TFB2M"*
**作者**：G.S. Shadel et al.
**摘要**：本研究通过重组表达纯化人源TFB2M蛋白，成功在体外重建了线粒体转录系统，证实TFB2M与线粒体RNA聚合酶(POLRMT)及TFB1M协同作用，显著增强mtDNA启动子特异性转录活性。
2. **文献名称**：*"Structural Basis of Mitochondrial Transcription Initiation: TFB2M as a Conformational Switch"*
**作者**：J. Sologub et al.
**摘要**：利用X射线晶体学解析重组TFB2M蛋白的三维结构，揭示其通过构象变化调控POLRMT的催化活性，阐明了TFB2M在转录起始复合物组装中的分子机制。
3. **文献名称**：*"Functional Analysis of TFB2M Mutations Linked to Mitochondrial Diseases Using Recombinant Protein Assays"*
**作者**：R. McWilliams et al.
**摘要**：通过重组TFB2M突变体蛋白的功能实验，发现致病突变(如p.R317Q)导致其与POLRMT的结合能力下降，破坏转录起始复合物稳定性，为线粒体疾病机制提供分子解释。
注：上述文献信息为示例性质，实际研究中建议通过PubMed或Google Scholar以“TFB2M recombinant protein”等关键词检索最新文献。
**Background of TFB2M Recombinant Protein**
TFB2M (Transcription Factor B2. Mitochondrial) is a nuclear-encoded protein critical for mitochondrial gene expression and energy metabolism. It functions as a key component of the mitochondrial transcription machinery, working alongside mitochondrial RNA polymerase (POLRMT) and Transcription Factor A (TFAM) to regulate the transcription and replication of mitochondrial DNA (mtDNA). TFB2M is structurally related to its paralog TFB1M, but unlike TFB1M, which primarily acts as an rRNA methyltransferase, TFB2M directly facilitates the assembly of the transcription initiation complex by stabilizing POLRMT and enhancing promoter recognition.
The recombinant TFB2M protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems, followed by purification to achieve high homogeneity. This recombinant form retains the functional properties of native TFB2M, enabling researchers to study its role in mtDNA transcription, mitochondrial biogenesis, and cellular energy production *in vitro* or in model organisms.
Studies involving TFB2M recombinant protein have shed light on mitochondrial dysfunction linked to diseases such as neurodegenerative disorders, metabolic syndromes, and aging. Its interaction with other mitochondrial transcription factors and potential regulatory mechanisms are also areas of active investigation. Additionally, recombinant TFB2M serves as a tool for drug screening aimed at modulating mitochondrial activity or treating mtDNA-related pathologies.
Overall, TFB2M recombinant protein is a vital reagent for dissecting mitochondrial transcription mechanisms and developing therapeutic strategies for mitochondrial diseases.
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