首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠诱导型一氧化氮合成酶(iNOS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NOS2含量。NOS2即诱导型一氧化氮合酶，能催化产生大量一氧化氮。在炎症、免疫反应等病理生理过程中起关键作用。研究机制方面，主要聚焦其调控通路、与疾病发生发展关联，旨在通过干预NOS2活性，为炎症性疾病、肿瘤等治疗提供新策略。试剂盒检测范围为7.8 IU/mL-500 IU/mL，适用于体外培养细胞、实验动物模型的iNOS表达检测，为炎症机制研究、药物作用靶点验证及免疫调控实验提供可靠工具，广泛应用于基础医学、药理学及生物医学研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nos2 ELISA Kit; InoslNitric oxide synthase ELISA Kit; inducible ELISA Kit; EC 1.14.13.39 ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; Macrophage NOS ELISA Kit; MAC-NOS ELISA Kit; NOS type II ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit
缩写：NOS2
Uniprot No.：P29477
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：7.8 IU/mL-500 IU/mL
灵敏度：1.95 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-107 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of mouse iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-105 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/ml.OD1OD2AverageCorrected5002.5992.5812.5902.4032502.0872.0962.0921.9051251.3411.3691.3551.16862.50.7600.7980.7790.59231.20.4990.4800.4900.30315.60.3440.3680.3560.1697.80.2820.2930.2880.10100.1860.1880.187 | ||||||
货期：3-5 working days
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