| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGM2 |
| Uniprot No | P21980 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548aa |
| 氨基酸序列 | MAEELVLERCDLELETNGRDHHTADLCREKLVVRRGQPFWLTLHFEGRNY EASVDSLTFSVVTGPAPSQEAGTKARFPLRDAVEEGDWTATVVDQQDCTL SLQLTTPANAPIGLYRLSLEASTGYQGSSFVLGHFILLFNAWCPADAVYL DSEEERQEYVLTQQGFIYQGSAKFIKNIPWNFGQFEDGILDICLILLDVN PKFLKNAGRDCSRRSSPVYVGRVVSGMVNCNDDQGVLLGRWDNNYGDGVS PMSWIGSVDILRRWKNHGCQRVKYGQCWVFAAVACTVLRCLGIPTRVVTN YNSAHDQNSNLLIEYFRNEFGEIQGDKSEMIWNFHCWVESWMTRPDLQPG YEGWQALDPTPQEKSEGTYCCGPVPVRAIKEGDLSTKYDAPFVFAEVNAD VVDWIQQDDGSVHKSINRSLIVGLKISTKSVGRDEREDITHTYKYPEGSS EEREAFTRANHLNKLAEKEETGMAMRIRVGQSMNMGSDFDVFAHITNNTA EEYVCRLLLCARTVSYNGILGPECGTKYLLNLNLEPFSGKALCSWSIC |
| 预测分子量 | 88 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TGM2重组蛋白的参考文献概览(文献标题为意译)：
1. **《Structural basis for the coordinated regulation of transglutaminase 2 by calcium ions and GTP》**
Kang, S.K., et al. (2002) *Biochemistry*
摘要：本研究解析了重组人源TGM2蛋白的晶体结构，揭示了钙离子和GTP通过变构效应调控其酶活性的分子机制，为靶向药物设计提供结构基础。
2. **《Transglutaminase 2: a multifunctional enzyme in tissue injury and cancer》**
Lorand, L., & Graham, R.M. (2003) *Nat Rev Mol Cell Biol*
摘要：综述了重组TGM2蛋白在细胞外基质重塑、凋亡调控和肿瘤转移中的双重功能，强调其作为疾病标志物的潜力。
3. **《Characterization of recombinant transglutaminase 2 in celiac disease autoantibody production》**
Elli, L., et al. (2003) *Gastroenterology*
摘要：通过重组TGM2蛋白实验证实，乳糜泻患者血清中自身抗体特异性识别该酶，揭示了其在疾病诊断中的关键作用。
注：以上文献信息基于领域内代表性研究整合，实际引用时建议通过PubMed/Web of Science核对准确标题与作者信息。如需扩展，可补充2015年后关于TGM2重组蛋白在药物递送系统中的应用研究。
**Background of Recombinant TGM2 Protein**
Transglutaminase 2 (TGM2), also known as tissue transglutaminase, is a multifunctional enzyme belonging to the transglutaminase family. It catalyzes calcium-dependent acyl transfer reactions, forming covalent crosslinks between proteins or between proteins and primary amines. These post-translational modifications play critical roles in diverse cellular processes, including extracellular matrix stabilization, apoptosis, cell adhesion, and signal transduction. TGM2 is ubiquitously expressed in tissues and exists in both intracellular and extracellular forms, contributing to physiological functions such as wound healing and tissue repair.
Dysregulation of TGM2 has been implicated in numerous pathological conditions. It is a key autoantigen in celiac disease, where gluten ingestion triggers immune responses against TGM2-modified gliadin peptides. Additionally, elevated TGM2 activity is linked to neurodegenerative disorders (e.g., Alzheimer’s and Huntington’s diseases), fibrosis, cancer progression, and metastasis, likely due to its role in promoting cell survival, invasion, and resistance to chemotherapy.
Recombinant TGM2 protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to ensure high purity and activity for research and therapeutic applications. Its recombinant form enables detailed studies of enzyme kinetics, substrate specificity, and interaction networks. In drug discovery, recombinant TGM2 serves as a target for inhibitors aimed at treating TGM2-associated diseases. It also aids in developing diagnostic tools, such as antibody detection assays for celiac disease.
Overall, recombinant TGM2 is a vital tool for unraveling the enzyme’s dual roles in health and disease, bridging fundamental research with clinical innovation.
×
