首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠notum蛋白同源物(NOTUM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的NOTUM含量。NOTUM是一个重要靶点。它是分泌性蛋白，可调节Wnt信号通路。异常激活的Wnt信号与多种疾病相关，如肿瘤、纤维化等。对NOTUM的研究，主要围绕其对Wnt信号的调控机制，期望通过干预该靶点为相关疾病治疗提供新思路。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于体外研究NOTUM在胚胎发育、干细胞分化以及骨代谢相关疾病模型中的表达动态，尤其适合评估不同处理条件下（如基因编辑、药物干预等）细胞或组织内源性NOTUM的分泌水平变化，为探索Wnt信号通路调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NotumPalmitoleoyl-protein carboxylesterase NOTUM ELISA Kit; EC 3.1.1.98 ELISA Kit
缩写：NOTUM
Uniprot No.：Q8R116
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 109 | ||||
| Range % | 104-113 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 96-104 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of mouse NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-89 | ||||
| EDTA plasma (n=4) | 98 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7792.7982.7892.58752.2012.1562.1791.9772.51.4841.4511.4681.2661.250.8450.8990.8720.6700.6250.5550.5610.5580.3560.3120.4320.4450.4390.2370.1560.3120.3260.3190.11700.2010.2030.202 | ||||||
货期：3-5 working days
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