首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠脑钠素/脑钠尿肽(BNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的NPPB含量。NPPB即利钠肽B前体，在心血管等系统中发挥重要生理作用，可调节体液平衡和血压。研究发现其与心力衰竭等疾病密切相关，通过其相关信号通路机制可深入了解疾病发生发展，有望为相关疾病的诊断、治疗提供新靶点和思路。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于心脑血管疾病动物模型研究、药物心脏安全性评价、心肌细胞功能实验等科研场景，可精准分析小鼠样本中BNP的动态变化，为心血管机制研究及生物标志物探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NppbNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide ELISA kit; BNP)] ELISA kit
缩写：NPPB
Uniprot No.：P40753
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 92 | |
| Range % | 86-98 | ||
| 1:2 | Average % | 96 | |
| Range % | 90-102 | ||
| 1:4 | Average % | 103 | |
| Range % | 100-107 | ||
| 1:8 | Average % | 89 | |
| Range % | 84-95 |
回收率：
| The recovery of mouse BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 90-95 | ||||
| EDTA plasma (n=4) | 103 | 101-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.8772.9842.9312.774 2002.1362.0152.0761.919 1001.3221.4331.3781.221 500.8110.8620.8370.680 250.5120.5140.5130.356 12.50.3220.3020.3120.155 6.250.2400.2500.2450.088 00.1530.1600.157 | ||||||
货期：3-5 working days
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