首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠NAD(P)H脱氢酶[醌]1(NQO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NQO1含量。NQO1即醌氧化还原酶1，是一种重要的抗氧化酶。它广泛存在于人体组织中，能参与醌类化合物代谢，在解毒、抗氧化等方面发挥关键作用。研究发现其与肿瘤发生发展密切相关，常被作为肿瘤治疗潜在靶点，通过调控它来干预肿瘤进程。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品为科研人员研究氧化应激相关分子机制、建立疾病模型或评估药物/化合物对NQO1调控作用提供可靠工具，特别适用于探索NQO1在代谢性疾病、癌症或神经保护中的功能，以及筛选影响该酶活性的天然产物或合成化合物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nqo1 ELISA Kit; Dia4 ELISA Kit; Nmo1 ELISA Kit; Nmor1 ELISA Kit; NAD(P)H dehydrogenase [quinone] 1 ELISA Kit; EC 1.6.5.2 ELISA Kit; Azoreductase ELISA Kit; DT-diaphorase ELISA Kit; DTD ELISA Kit; Menadione reductase ELISA Kit; NAD(P)H:quinone oxidoreductase 1 ELISA Kit; Phylloquinone reductase ELISA Kit; Quinone reductase 1 ELISA Kit; QR1 ELISA Kit
缩写：NQO1
Uniprot No.：Q64669
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NQO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-97 | |||||
回收率：
| The recovery of mouse NQO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 86-98 | ||||
| EDTA plasma (n=4) | 99 | 94-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7052.6002.6532.49810002.3042.3712.3382.1835001.6591.7581.7091.5542500.7940.7950.7950.6401250.4950.5220.5090.35462.50.3520.3570.3550.20031.250.2070.2240.2160.06100.1500.1600.155 | ||||||
货期：3-5 working days
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