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产品描述：犬N端前脑钠素(NT-proBNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的NT-proBNP含量。NT - proBNP是脑钠肽原裂解后无活性的N端片段。其水平与心室壁张力及容量负荷密切相关，因此可作为反映心脏功能的生物标志物。临床上常用于心力衰竭的诊断、病情评估及预后判断，在心血管疾病研究中是重要靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于犬血清或血浆样本中心脏功能相关生物标志物的科研分析；常用于评估犬类心肌肥厚、充血性心力衰竭等疾病的病理机制研究；支持科研人员开展心血管疾病模型构建、药物干预效果评估或病理生理机制研究，为犬类心脏疾病的实验动物模型提供可靠的分子检测工具；适用于实验室常规检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NT-proBNP
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of canine NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-108 | ||||
| EDTA plasma (n=4) | 97 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.6752.6552.6652.516 102.1522.1442.1481.999 51.3111.3021.3071.158 2.50.7810.7990.7900.641 1.250.3770.3630.3700.221 0.6250.2640.2540.2590.110 0.3120.1860.1900.1880.039 00.1460.1520.149 | ||||||
货期：3-5 working days
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