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产品描述：牛干扰素-tau酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFN-tau含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，支持科研人员对牛繁殖生物学、妊娠相关疾病机制及胚胎 - 母体互作等领域的研究。其宽泛的检测范围可精准量化生理及病理状态下样本中 IFN - τ 的动态变化，为评估胚胎活力、优化繁殖效率及探究妊娠相关分子调控网络提供可靠工具，适用于畜牧科学、生殖医学及比较生物学等基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNT1 ELISA Kit; Interferon tau-1 ELISA Kit; IFN-tau-1 ELISA Kit; Antiluteolysin ELISA Kit; Trophoblast antiluteolytic protein ELISA Kit; Trophoblast protein 1 ELISA Kit; TP-1 ELISA Kit; Trophoblastin ELISA Kit
缩写：IFN-tau
Uniprot No.：P15696
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine interferon-tau in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-101 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of bovine interferon-tau spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-96 | ||||
| EDTA plasma (n=4) | 96 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.5872.5582.5732.4852002.1402.0032.0721.9841001.6441.6211.6331.545501.1091.0271.0680.980250.6980.6870.6930.60512.50.3990.3870.3930.3056.250.2420.2310.2370.14900.0900.0860.088 | ||||||
货期：3-5 working days
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