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产品描述：鸡饥饿激素酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Ghrelin含量。Ghrelin是一种重要靶点。它是胃内产生的肽，能调节食欲和能量稳态。研究机制方面，Ghrelin与受体结合后，作用于神经内分泌系统，影响下丘脑信号通路，调节摄食、生长激素释放等，在肥胖、代谢疾病等研究中受关注。试剂盒检测范围为25 pg/mL-400 pg/mL，适用于家禽生理学研究、营养代谢实验、生长调控机制探索等科研领域，如评估饲料成分对Ghrelin分泌的影响或分析激素水平与生长性能的关联性，为禽类生物学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Ghrelin
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-400 pg/mL
灵敏度：25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken ghrelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of chicken ghrelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-98 | ||||
| EDTA plasma (n=4) | 93 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 4000.2480.2440.246 2000.5060.5000.503 1000.8630.8560.860 501.6871.7021.695 252.5152.4232.469 | ||||||
货期：3-5 working days
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