首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨特性碱性磷酸酶(BALP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BALP含量。BALP即骨碱性磷酸酶，是成骨细胞的特异性标志物。其背景在于反映骨代谢情况，在骨骼生长、修复等过程有重要意义。研究机制主要是通过检测其活性，以评估骨转换状态，辅助诊断儿童佝偻病、骨质疏松等骨代谢相关疾病。试剂盒检测范围为1.25 ng/mL-80 ng/mL，该产品可广泛应用于骨骼发育研究、骨质疏松模型构建、骨修复材料效果评估等科研领域，尤其适用于分析药物干预或基因修饰对骨代谢影响的实验中。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpl ELISA Kit; Akp-2 ELISA Kit; Akp2 ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase 2 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit
缩写：BALP
Uniprot No.：P09242
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:10 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:20 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:40 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of mouse BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-98 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.9122.8112.8622.707402.3212.2032.2622.107201.4681.4471.4581.303100.8770.8660.8720.71750.5220.5340.5280.3732.50.3500.3620.3560.2011.250.2590.2670.2630.10800.1540.1550.155 | ||||||
货期：3-5 working days
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