首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠儿茶酚抑素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的catestatin含量。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于体外培养细胞模型中CST分泌水平的动态监测、疾病动物模型（如高血压或心肌缺血模型）组织内源性CST表达分析，以及探索CST在代谢综合征相关机制中的作用，为神经内分泌调控、心血管病理机制等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：catestatin
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse catestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 92 | |
| Range % | 87-96 | ||
| 1:2 | Average % | 99 | |
| Range % | 95-103 | ||
| 1:4 | Average % | 101 | |
| Range % | 94-106 | ||
| 1:8 | Average % | 95 | |
| Range % | 89-98 |
回收率：
| The recovery of mouse catestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-98 | ||||
| EDTA plasma (n=4) | 99 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.9893.0022.9962.853 25002.5472.4442.4962.353 12501.9841.9981.9911.848 6251.2581.1681.2131.070 3120.6550.6430.6490.506 1560.5020.4990.5010.358 780.3060.3250.3160.173 00.1380.1470.143 | ||||||
货期：3-5 working days
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