Mouse Desmosine ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E14196m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：小鼠锁链素酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Desmosine含量。Desmosine是一种交联氨基酸，主要存在于弹性蛋白中。其背景在于它是弹性纤维结构稳定的关键成分。研究机制上，常作为评估弹性蛋白降解的标志物，通过检测其含量变化，帮助了解与弹性蛋白异常降解相关疾病的发病进程等。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研场景下的小鼠模型研究，如评估疾病进程中弹性蛋白代谢异常、验证药物对组织修复的影响或探索衰老相关分子机制，常用于研究肺部纤维化、慢性阻塞性肺病（COPD）及皮肤老化等相关机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Desmosine
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Desmosine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	85
	Range %	81-92
1:20	Average %	96
	Range %	91-103
1:40	Average %	103
	Range %	97-107
1:80	Average %	98
	Range %	92-102

回收率：

The recovery of mouse Desmosine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-96
EDTA plasma (n=4)	104	96-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected2002.4952.5942.5452.3551001.8041.9031.8541.664501.1281.1481.1380.948250.6700.6610.6660.47612.50.4410.4220.4320.2426.250.3140.3050.3100.1203.120.2550.2570.2560.06600.1890.1900.190