首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Cramp酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CAMP含量。CAMP，即环磷酸腺苷，是细胞内重要的第二信使。它广泛参与细胞多种生理过程的信号传导。其研究机制主要围绕在激素等信号刺激下，通过激活腺苷酸环化酶生成，进而调节蛋白激酶活性，影响下游基因表达和细胞功能等。试剂盒检测范围为1.56 pg/mL-100 pg/mL，本产品适用于基础医学研究、药物开发等领域的体外实验，特别针对免疫相关动物模型的组织微环境分析、炎症因子调控机制探索等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Camp ELISA Kit; Cnlp ELISA Kit; CrampCathelicidin antimicrobial peptide ELISA Kit; Cathelin-like protein ELISA Kit; CLP) [Cleaved into: Cathelin-related antimicrobial peptide ELISA Kit; Cramp)] ELISA Kit
缩写：CAMP
Uniprot No.：P51437
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse cramp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse cramp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-94 | ||||
| EDTA plasma (n=4) | 102 | 94-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.3002.2002.2502.154501.5051.4051.4551.359250.8220.8020.8120.71612.50.4450.4350.4400.3446.250.3140.3040.3090.2133.120.2110.2010.2060.1101.560.1490.1470.1480.05200.0970.0950.096 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse CRAMP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CRAMP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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