首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠高密度脂蛋白(HDL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HDL含量。HDL即高密度脂蛋白，它具有抗动脉粥样硬化作用，在胆固醇逆向转运等过程中发挥关键作用。研究聚焦其提升机制，比如激活相关信号通路、调节关键酶活性，以提高HDL水平和功能，进而防治心血管疾病。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，适用于科研领域中对小鼠模型脂代谢机制、心血管疾病病理研究或药物干预效果的评价；为探索HDL在生理及疾病状态下的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HDL
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.8 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2000 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4000 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8000 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of mouse HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4592.4692.4642.33610001.9621.9961.9791.8515001.4151.4271.4211.2932500.9550.9680.9620.8341250.5690.5380.5540.42662.50.3390.3260.3330.20531.250.2740.2540.2640.13600.1290.1270.128 | ||||||
货期：3-5 working days
×
