首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人阿立新A(Orexin A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Orexin A含量。Orexin A，又称食欲素A，是一种由下丘脑神经元分泌的神经肽，主要作用是调节觉醒、睡眠和食欲。其通过激活OX1R和OX2R受体，影响神经递质系统，如GABA和谷氨酸，从而调节睡眠-觉醒周期和能量代谢。研究显示，Orexin A在治疗睡眠障碍和调节食欲方面具有潜在应用价值。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中探索Orexin A在神经系统功能、代谢调控机制以及相关疾病动物模型中的作用，例如通过检测不同生理状态或药物干预下生物样本中Orexin A的动态变化，为神经生物学、内分泌学及行为学研究提供定量分析工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hcrt ELISA Kit; Hcrt1 ELISA Kit; Hcrt2 ELISA Kit; Hypocretin ELISA Kit; Hypocretin neuropeptide precursor ELISA Kit; Hypocretin-1 ELISA Kit; Hypocretin-2 ELISA Kit; OREX_HUMAN ELISA Kit; Orexin ELISA Kit; Orexin precursor ELISA Kit; Orexin-B ELISA Kit; OX ELISA Kit; PPOX ELISA Kit
缩写：Orexin A
Uniprot No.：O43612
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-86 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 100-106 | |||||
回收率：
| The recovery of human orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-96 | ||||
| EDTA plasma (n=4) | 97 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.3342.5562.4452.34152.1612.1752.1682.0642.51.6451.7561.7011.5971.251.2571.2671.2621.1580.6250.8180.8860.8520.7480.3120.5470.5680.5580.4540.1560.3310.3560.3440.24000.1020.1060.104 | ||||||
货期：3-5 working days
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