首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠破骨细胞相关免疫球蛋白样受体(OSCAR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OSCAR含量。小鼠破骨细胞相关免疫球蛋白样受体(OSCAR)在破骨细胞形成和免疫调节中起关键作用，能促进破骨细胞形成及存活，并参与免疫细胞的激活和成熟，是骨重建和免疫治疗的重要靶点。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，支持科研人员开展骨代谢调控机制、骨质疏松模型评估、骨免疫相关药物开发等基础研究，为体外实验提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Oscar ELISA Kit; Osteoclast-associated immunoglobulin-like receptor ELISA Kit; Osteoclast-associated receptor ELISA Kit; mOSCAR ELISA Kit; Ocl178 ELISA Kit
缩写：OSCAR
Uniprot No.：Q8VBT3
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：37.5 pg/mL-2400 pg/mL
灵敏度：9.375 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSCAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:5 | Average % | 89 | |
| Range % | 82-93 | ||
| 1:10 | Average % | 105 | |
| Range % | 97-108 | ||
| 1:20 | Average % | 93 | |
| Range % | 88-97 | ||
| 1:40 | Average % | 95 | |
| Range % | 91-98 |
回收率：
| The recovery of mouse OSCAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 91 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 24002.8852.8772.8812.761 12002.0682.0892.0791.959 6001.1521.1231.1381.018 3000.6890.6870.6880.568 1500.3980.3910.3950.275 750.3010.2890.2950.175 37.50.2100.2220.2160.096 00.1190.1210.120 | ||||||
货期：3-5 working days
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