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产品描述：牛α1酸性糖蛋白(α1-AGP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ORM1含量。ORM1 即阿片样物质 μ 型受体 1，是一种与阿片类物质作用相关的靶点。其背景上在痛觉调节等生理过程有重要地位。研究机制方面，主要围绕其与配体结合，通过信号转导通路发挥效应，在镇痛药物研发等领域是重要研究对象。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，该产品适用于科研领域，如评估牛类感染性疾病模型、炎症相关药效学研究、营养代谢对免疫系统的影响，或通过组织匀浆分析特定器官在病理条件下的蛋白表达特征。为畜牧兽医研究、动物健康监测及基础医学机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ORM1 ELISA kit; AGPAlpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
缩写：ORM1
Uniprot No.：Q3SZR3
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 102 | ||||
| Range % | 94-106 | |||||
| 1:2000 | Average % | 92 | ||||
| Range % | 84-97 | |||||
| 1:4000 | Average % | 101 | ||||
| Range % | 95-104 | |||||
| 1:8000 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of bovine α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 88-99 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 40000.2290.2250.227 20000.4500.4590.455 10000.6980.6560.677 5001.0160.9871.002 2501.2641.2431.254 1251.5721.5141.543 62.51.6711.6341.653 01.8151.7891.802 | ||||||
货期：3-5 working days
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