首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠阿立新A(Orexin A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的Orexin A含量。Orexin A是一种神经肽。其背景在于它在调节觉醒与食欲等方面有重要作用。研究机制上，它能与特异性受体结合，激活细胞内信号通路，影响神经活动，进而调控生理功能，在睡眠障碍、代谢疾病等领域是研究热点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于神经生物学研究、代谢调控机制探索或睡眠 - 觉醒周期相关实验中的目标蛋白定量分析。科研人员可通过评估Orexin A在小鼠中枢神经系统疾病模型、能量代谢异常研究或药理干预实验中的表达水平变化，为神经肽相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hcrt ELISA Kit; Ox ELISA Kit; Ppox ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit
缩写：Orexin A
Uniprot No.：O55241
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 94-107 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 82-98 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 96-108 | ||||||
回收率：
| The recovery of mouse orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 96-109 | |||||
| EDTA plasma (n=4) | 100 | 89-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected50002.4892.4132.4512.28425002.0391.9822.0111.84412501.4611.5001.4811.3146251.0641.0181.0410.8743120.6930.6660.6800.5131560.5080.5250.5170.350780.3040.3140.3090.14200.1680.1660.167 | |||||||
货期：3-5 working days
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