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产品描述：人抑瘤素M(OSM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的OSM含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，灵敏度为1.56 pg/mL。本试剂盒适用于体外科研场景，例如探究OSM在肿瘤细胞与免疫细胞互作中的调控机制、评估炎症性疾病模型中OSM的表达动态，或分析药物处理对细胞分泌OSM水平的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MGC20461 ELISA Kit; ONCM_HUMAN ELISA Kit; Oncostatin M ELISA Kit; Oncostatin-M ELISA Kit; OSM ELISA Kit
缩写：OSM
Uniprot No.：P13725
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OSM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 83-97 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 81-95 | ||||||
回收率：
| The recovery of human OSM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 94-102 | |||||
| EDTA plasma (n=4) | 107 | 100-115 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 4001.7791.8441.8121.642 2001.0111.0231.0170.847 1000.6850.6660.6760.506 500.4210.4150.4180.248 250.2960.3020.2990.129 12.50.2510.2650.2580.088 6.250.2220.2320.2270.057 00.1650.1740.170 | |||||||
货期：3-5 working days
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