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产品描述：小鼠III型前胶原氨基末端肽(PⅢNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PⅢNP含量。PⅢNP（Ⅲ型前胶原氨基端肽）是一种纤维化指标，其水平与心室重塑程度相关，对评估慢性心力衰竭（CHF）的严重程度有重要指导意义。研究发现，PⅢNP水平与Gal-3（半乳糖凝集素3）水平呈显著正相关，提示PⅢNP可能通过促进组织纤维化参与CHF的发生发展。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外研究纤维化疾病模型中的胶原代谢动态、评估抗纤维化药物干预效果，以及干细胞治疗或组织工程领域中对胶原合成活性的监测，为探索纤维化发病机制及治疗策略提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N-terminal procollagen 3 propeptide(P3NP),_x000D_ N-terminal procollagen III propeptide(PIIINP)
缩写：PⅢNP
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PIIINP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-97 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-108 | |||||
回收率：
| The recovery of mouse PIIINP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.5692.4992.5342.381 101.7981.7681.7831.630 51.2981.2461.2721.119 2.50.8540.8980.8760.723 1.250.4740.4980.4860.333 0.6250.3710.3980.3850.232 0.3120.2220.2330.2280.075 00.1520.1540.153 | ||||||
货期：3-5 working days
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