首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠纤溶酶抗纤溶酶复合物(PAP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PAP含量。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于体外实验环境下检测小鼠模型中与血栓形成、溶栓治疗或炎症反应相关的PAP动态变化，例如在血栓性疾病模型评估、抗凝药物作用机制研究或纤溶系统调控实验中定量分析样本生物标志物水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：PAP
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 87 | ||||
| Range % | 83-94 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:20 | Average % | 105 | ||||
| Range % | 98-111 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 101 | 95-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.2452.2642.2552.0721001.8051.8551.8301.647501.1781.1551.1670.984250.7390.7960.7680.58512.50.3980.4210.4100.2276.250.3120.3350.3240.1413.120.2590.2480.2540.07100.1880.1780.183 | ||||||
货期：3-5 working days
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