首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒

Mouse Phenylalanine-4-hydroxylase(PAH) ELISA kit

  • 中文名： 小鼠苯丙氨酸羟化酶4(PAH)酶联免疫试剂盒
货号: IPD-EL017396MO
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：小鼠苯丙氨酸羟化酶4(PAH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PAH含量。PAH（苯丙氨酸羟化酶）是参与苯丙氨酸代谢的关键酶。其基因突变会导致苯丙酮尿症等疾病。研究机制主要聚焦于解析其结构与功能关系，探索恢复或增强酶活性的方法，以开发治疗相关疾病的药物和疗法。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于实验室环境下探究PAH在小鼠模型中的表达水平变化，如代谢通路研究、基因编辑动物模型的表型验证、药物干预对酶活性影响的评估等科研场景。可为细胞生物学、分子病理学及基础医学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Pah ELISA Kit; Phenylalanine-4-hydroxylase ELISA Kit; PAH ELISA Kit; EC 1.14.16.1 ELISA Kit; Phe-4-monooxygenase ELISA Kit

  • 缩写：PAH

  • Uniprot No.：P16331

  • 种属：Mus musculus (Mouse)

  • 样本类型：serum, plasma, tissue homogenates, cell lysates

  • 检测范围：23.5 pg/mL-1500 pg/mL

  • 灵敏度：5.8 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Metabolism

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %104

    Range %101-109

    1:2Average %95

    Range %91-99

    1:4Average %86

    Range %83-89

    1:8Average %92

    Range %87-96

  • 回收率：

    The recovery of mouse PAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)8683-89

    EDTA plasma (n=4)8985-96

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected15002.6462.7012.6742.4817502.0632.0002.0321.8393751.3241.4051.3651.172187.50.8260.8340.8300.637940.5930.5870.5900.397470.3620.3710.3670.17423.50.2780.2840.2810.08800.1920.1940.193　







  • 货期：3-5 working days

客户数据及评论

折叠内容
默认 最早 支持最多 评分最高
0条

大包装询价

×