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产品描述：小鼠精氨酸脱亚胺酶4(PADI4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PADI4含量。PADI4 是一种颇受关注的靶点。它在多种生理和病理过程中发挥作用。研究发现其参与蛋白质瓜氨酸化过程，与自身免疫性疾病、肿瘤的发生发展等机制相关。对 PADI4 的深入研究有助于揭示疾病发病机理，为相关疾病治疗提供新方向。试剂盒检测范围为4.7 pg/mL-300 pg/mL，适用于体外实验，能够支持多种研究场景，如探索炎症性疾病模型中PADI4的表达动态、评估基因编辑或药物干预后酶活性变化，或分析肿瘤微环境中蛋白质翻译后修饰机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Padi4 ELISA Kit; Pad4 ELISA Kit; Pdi4 ELISA Kit; Protein-arginine deiminase type-4 ELISA Kit; EC 3.5.3.15 ELISA Kit; Peptidylarginine deiminase IV ELISA Kit; Protein-arginine deiminase type IV ELISA Kit
缩写：PADI4
Uniprot No.：Q9Z183
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PADI4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of mouse PADI4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 88-105 | ||||
| EDTA plasma (n=4) | 96 | 89-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected3002.2282.2302.2292.0511501.4571.3781.4181.240750.8540.8220.8380.66037.50.4830.5130.4980.32018.750.3550.3630.3590.1819.40.2740.2590.2670.0894.70.2330.2260.2300.05200.1750.1810.178 | ||||||
货期：3-5 working days
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