首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠蛋白质二硫键异构酶(P4HB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的P4HB含量。P4HB 即脯氨酰 4-羟化酶β亚基，是内质网内的多功能蛋白。其参与胶原蛋白合成，对维持细胞外基质稳定至关重要。在研究机制上，它作为伴侣蛋白促进蛋白质折叠，还参与氧化还原反应。P4HB 与多种疾病相关，是潜在的治疗靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，为研究蛋白质折叠调控机制、氧化应激相关疾病模型（如肝脏疾病或代谢异常）以及内质网功能相关分子通路提供可靠工具；适用于体外实验中探索P4HB在细胞生理病理过程中的表达变化及其分子互作研究，满足基础科研中对目标蛋白定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：P4hb ELISA Kit; Pdia1Protein disulfide-isomerase ELISA Kit; PDI ELISA Kit; EC 5.3.4.1 ELISA Kit; Cellular thyroid hormone-binding protein ELISA Kit; Endoplasmic reticulum resident protein 59 ELISA Kit; ER protein 59 ELISA Kit; ERp59 ELISA Kit; Prolyl 4-hydroxylase subunit beta ELISA Kit; p55 ELISA Kit
缩写：P4HB
Uniprot No.：P09103
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse P4HB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 96-108 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 95-107 | ||||||
回收率：
| The recovery of mouse P4HB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-95 | |||||
| EDTA plasma (n=4) | 99 | 91-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.2482.1752.2122.044101.6151.5841.6001.43251.0160.9670.9920.8242.50.5450.5360.5410.3731.250.3420.3580.3500.1820.6250.2510.2430.2470.0790.3120.2150.2100.2130.04500.1710.1650.168 | |||||||
货期：3-5 working days
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