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产品描述：犬前白蛋白(PA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PA含量。PA（靶点）是具有重要研究价值的靶点。在它可能与特定疾病相关，成为药物研发关注焦点。研究机制上，科研人员或通过解析其结构、功能及与其他分子相互作用，探寻调节途径，为开发针对性药物、治疗相应疾病奠定基础。试剂盒检测范围为0.625 μg/mL-10 μg/mL，适用于犬类肝脏功能研究、营养干预实验、疾病模型构建等科研领域，尤其适合探究慢性肝病、代谢综合征或药物毒性对蛋白质合成的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PA
Uniprot No.：E2R5U8
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 μg/mL-10 μg/mL
灵敏度：0.156 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 99-106 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of canine PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-105 | ||||
| EDTA plasma (n=4) | 89 | 84-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 100.1450.1390.142 50.2750.2620.269 2.50.5120.5500.531 1.250.9130.9830.948 0.6251.6651.5601.613 02.6472.4322.540 | ||||||
货期：3-5 working days
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