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产品描述:人免疫抑制性糖蛋白酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、脑脊液(CSF)、尿液、组织匀浆样本中的PAEP含量。PAEP 即妊娠-associated endometrial protein,在生殖生理等方面有重要作用。研究发现它与子宫内膜容受性等过程相关,还可能参与免疫调节,维持母胎免疫耐受。其具体机制可能涉及信号通路激活、蛋白相互作用,为生殖领域研究提供重要方向。试剂盒检测范围为0.78 ng/mL-50 ng/mL,该产品为研究免疫抑制机制、肿瘤微环境调控、自身免疫性疾病或感染性疾病中的免疫逃逸现象提供有效工具,适用于体外实验中对多类型生物样本的靶蛋白水平动态监测、药物干预效果评估及分子通路关联性分析等科研需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Alpha uterine protein ELISA Kit; gD ELISA Kit; GdA ELISA Kit; GdF ELISA Kit; GdS ELISA Kit; Glycodelin A ELISA Kit; Glycodelin ELISA Kit; Glycodelin F ELISA Kit; Glycodelin S ELISA Kit; MGC138509 ELISA Kit; MGC142288 ELISA Kit; PAEG ELISA Kit; PAEP ELISA Kit; PAEP_HUMAN ELISA Kit; PEG ELISA Kit; PEP ELISA Kit; Placental Protein 14 / Glycodelin A ELISA Kit; Placental protein 14 ELISA Kit; PP14 ELISA Kit; Pregnancy-associated endometrial alpha-2 globulin ELISA Kit; Progestagen-associated endometrial protein ELISA Kit; Progesterone-associated endometrial protein ELISA Kit
缩写:PAEP
Uniprot No.:P09466
种属:Homo sapiens (Human)
样本类型:serum, cerebrospinal fluid (CSF), urine, tissue homogenates
检测范围:0.78 ng/mL-50 ng/mL
灵敏度:0.195 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human glycodelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 86 | ||||
Range % | 82-92 | |||||
1:2 | Average % | 99 | ||||
Range % | 94-105 | |||||
1:4 | Average % | 103 | ||||
Range % | 92-110 | |||||
1:8 | Average % | 91 | ||||
Range % | 86-96 |
回收率:
The recovery of human glycodelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 115 | 110-120 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected502.8212.6872.7542.631252.2872.1932.2402.11712.51.6421.5741.6081.4856.250.9640.9310.9480.8253.120.5810.5630.5720.4491.560.3890.3820.3860.2630.780.2650.2570.2610.13800.1250.1210.123 |
货期:3-5 working days
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