首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠DJ-1蛋白(PARK7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PARK7含量。PARK7 是一个重要靶点。它在帕金森病等神经退行性疾病中扮演关键角色，其编码的蛋白具有抗氧化、分子伴侣等功能。研究围绕其在神经细胞内的作用机制，如调节氧化应激、蛋白质稳态等展开，有望为相关疾病治疗提供新方向。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研领域中对小鼠模型或细胞实验中DJ-1蛋白表达水平的动态监测，例如神经退行性疾病机制研究、氧化应激相关信号通路探索、基因敲除/过表达模型的验证等，为体外实验和动物实验提供可靠的蛋白定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Park7 ELISA Kit; Protein/nucleic acid deglycase DJ-1 ELISA Kit; EC 3.1.2.- ELISA Kit; EC 3.5.1.- ELISA Kit; EC 3.5.1.124 ELISA Kit; Maillard deglycase ELISA Kit; Parkinson disease protein 7 homolog ELISA Kit; Parkinsonism-associated deglycase ELISA Kit; Protein DJ-1 ELISA Kit; DJ-1 ELISA Kit
缩写：PARK7
Uniprot No.：Q99LX0
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PARK7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-102 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-100 | |||||
回收率：
| The recovery of mouse PARK7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-102 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40003.0573.1043.0812.91120002.5772.4722.5252.35510001.6691.6831.6761.5065000.9421.0000.9710.8012500.5550.5750.5650.3951250.3900.4150.4030.23362.50.2830.3020.2930.12300.1690.1710.170 | ||||||
货期：3-5 working days
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