Mouse paired box 6 (PAX6) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL017492MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：小鼠成对盒蛋白6 (PAX6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PAX6含量。PAX6 是一个重要的靶点。它在胚胎发育尤其是眼睛、神经系统等器官的发育中起关键作用。研究机制上，其作为转录因子能调控众多下游基因表达。相关研究聚焦于其在发育异常及疾病中的变化，探索以此为靶点治疗相关病症的可能性。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，该产品适用于基础研究领域，例如在基因编辑小鼠模型中评估PAX6表达水平，研究其在神经发育、视网膜形成中的功能机制，或用于体外实验中探究药物、环境因素对PAX6调控的影响，为发育生物学、疾病机制研究提供可靠的工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pax6 ELISA Kit; Pax-6 ELISA Kit; Sey ELISA Kit; Paired box protein Pax-6 ELISA Kit; Oculorhombin ELISA Kit
缩写：PAX6
Uniprot No.：P63015
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAX6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-103
1:2	Average %	95
	Range %	89-99
1:4	Average %	101
	Range %	97-105
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of mouse PAX6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	98	93-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected30002.6622.5122.5872.42015002.2062.2602.2332.0667501.8401.7641.8021.6353751.2561.2291.2431.076187.50.6140.6670.6410.47493.750.4310.4060.4190.25246.880.2850.2710.2780.11100.1620.1720.167