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产品描述：犬降钙素原(PCT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的PCT含量。PCT即降钙素原，在健康人体中含量极低。细菌感染尤其是严重的全身感染时，PCT水平会显著升高。其研究机制是细菌内毒素等可刺激多种细胞产生PCT，临床上常通过检测其数值辅助判断是否存在细菌感染、评估感染严重程度及预后。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，在科研领域，该产品可用于探索犬类感染性疾病模型的病理机制、评估抗生素治疗过程中炎症反应动态变化，或作为免疫调节药物开发的生物标志物检测工具，为兽医基础研究及实验动物科学提供可靠的定量分析解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PCT
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 83-90 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 83 | |||||
| Range % | 80-86 | ||||||
回收率：
| The recovery of canine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 84-93 | |||||
| EDTA plasma (n=4) | 104 | 101-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.8392.9012.8702.77215002.3412.3022.3222.2247501.6021.6571.6301.5323750.9200.9720.9460.848187.50.4250.4080.4170.31993.750.2520.2610.2570.15946.880.1440.1520.1480.05000.0990.0960.098 | |||||||
货期：3-5 working days
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