首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠磷酸烯醇式丙酮酸羧激酶(PCK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PCK1含量。试剂盒检测范围为1.56 pg/mL-100 pg/mL，为研究能量代谢通路、肝脏功能及相关疾病模型提供可靠工具，广泛应用于基因敲除/过表达动物模型的验证、药物干预后代谢指标分析及分子机制探索等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pck1 ELISA Kit; Pepck ELISA Kit; Phosphoenolpyruvate carboxykinase ELISA Kit; cytosolic [GTP] ELISA Kit; PEPCK-C ELISA Kit; EC 4.1.1.32 ELISA Kit
缩写：PCK1
Uniprot No.：Q9Z2V4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:200 | Average % | 88 | |||||
| Range % | 84-93 | ||||||
| 1:400 | Average % | 101 | |||||
| Range % | 97-106 | ||||||
| 1:800 | Average % | 85 | |||||
| Range % | 80-91 | ||||||
回收率：
| The recovery of mouse PCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 100-105 | |||||
| EDTA plasma (n=4) | 89 | 85-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 1002.0362.1012.0691.875 501.6311.6021.6171.423 251.1081.2011.1550.961 12.50.7890.7600.7750.581 6.250.4850.4910.4880.294 3.120.3450.3390.3420.148 1.560.2750.2810.2780.084 00.1910.1960.194 | |||||||
货期：3-5 working days
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