首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子血小板衍生生长因子AB(PDGF-AB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AB含量。PDGF-AB是一种血小板衍生生长因子二聚体。其在细胞增殖、分化等生理过程及肿瘤、纤维化等疾病发生发展中起作用。研究机制上，主要通过与受体结合激活下游信号通路，影响细胞行为，目前是疾病治疗靶点研究热点之一。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外实验环境下对生物样本的批量检测，支持细胞生物学、基础医学研究中涉及组织再生、血管病变或肿瘤微环境等领域的 PDGF - AB 动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-AB
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 83-95 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 96-105 | |||||
回收率：
| The recovery of rabbit PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 83-93 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.8462.7342.7902.696502.1472.0352.0911.997251.2931.2481.2711.17712.50.6880.6770.6830.5896.250.4120.4240.4180.3243.120.2780.2890.2840.1901.560.1510.1530.1520.05800.0930.0940.094 | ||||||
货期：3-5 working days
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