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Mouse Programmed Death 1(PD-1)ELISA Kit

  • 中文名： 小鼠程序性死亡-1(PD-1)ELISA Kit
货号: IPD-E13586m
Price： ￥2500
数量：
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产品详情

  • 产品描述：小鼠程序性死亡-1(PD-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PDCD1含量。PDCD1即程序性死亡受体1，其基因编码的蛋白是一种重要的免疫检查点分子。它在T细胞活化后表达上调，可与配体结合抑制T细胞活性，避免免疫系统过度激活。当前在抗肿瘤研究中备受关注，通过阻断其信号通路可增强免疫细胞对肿瘤的杀伤作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，特别适用于免疫调节机制研究、肿瘤微环境分析、药物干预效果评估等科研场景，为PD - 1/PD - L1信号通路相关的基础研究提供可靠检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Pdcd1 ELISA Kit; Pd1 ELISA Kit; Programmed cell death protein 1 ELISA Kit; Protein PD-1 ELISA Kit; mPD-1 ELISA Kit; CD antigen CD279 ELISA Kit

  • 缩写：PDCD1

  • Uniprot No.：Q02242

  • 种属：Mus musculus (Mouse)

  • 样本类型：serum, plasma, tissue homogenates, cell lysates

  • 检测范围：0.312 ng/mL-20 ng/mL

  • 灵敏度：0.078 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Cell Biology

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PD-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %97

    Range %92-101

    1:2Average %91

    Range %85-96

    1:4Average %84

    Range %80-88

    1:8Average %95

    Range %95-99

  • 回收率：

    The recovery of mouse PD-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)10196-107

    EDTA plasma (n=4)9590-100

















    标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/mlOD1OD2AverageCorrected202.6522.5282.5902.430102.1212.0092.0651.90551.4391.4171.4281.2682.50.9940.9520.9730.8131.250.6510.6800.6660.5060.6250.3840.3750.3800.2200.3120.2560.2610.2590.09900.1630.1560.160　






  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PD-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled PD-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

  • 本试剂盒不含材料：



    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

    • An incubator can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：3-5 working days

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