首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠降钙素原(PCT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PCT含量。降钙素原（PCT）是一种蛋白质。在健康人体中，PCT 水平极低。当发生严重细菌、真菌、寄生虫感染及脓毒症和多脏器功能衰竭时，其水平会显著升高。研究认为，它可作为炎症和感染的生物标志物，辅助医生判断病情和指导治疗。试剂盒检测范围为1.56 pg/mL-100 pg/mL，可应用于科研场景中PCT相关机制研究。科研人员可将其应用于实验动物模型中脓毒症、全身性感染及炎症性疾病的机制探索，或用于评估药物干预对炎症通路的影响。本试剂盒支持多种生物样本类型检测，为实验室开展PCT动态监测、病原体 - 宿主互作研究及免疫调节实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：PCT
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 88-93 | |||||
| 1:40 | Average % | 102 | ||||
| Range % | 99-104 | |||||
| 1:80 | Average % | 94 | ||||
| Range % | 89-96 | |||||
| 1:160 | Average % | 97 | ||||
| Range % | 94-101 | |||||
回收率：
| The recovery of mouse PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 96 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.2242.3042.2642.090501.6551.6851.6701.496251.1221.1571.1400.96612.50.7320.7210.7270.5536.250.4410.4240.4330.2593.120.3150.3020.3090.1351.560.2420.2280.2350.06100.1760.1720.174 | ||||||
货期：3-5 working days
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