首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板衍生生长因子AB(PDGF-AB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AB含量。PDGF - AB是一种重要的靶点。它属于血小板衍生生长因子家族，在细胞生长、增殖、迁移和血管生成等过程中有关键作用。目前研究聚焦于其信号通路机制，探索通过阻断该靶点来抑制肿瘤生长、纤维化等疾病发展，为相关疾病治疗提供新思路。试剂盒检测范围为7.8 pg/mL-500 pg/mL，特别适用于大鼠模型研究中PDGF - AB的动态监测，例如创伤修复实验、炎症反应评估或血管生成相关机制研究，为探索PDGF - AB在组织再生、病理进程中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-AB
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:2 | Average % | 88 | |||||
| Range % | 84-93 | ||||||
| 1:4 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 95-106 | ||||||
回收率：
| The recovery of rat PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 83-90 | |||||
| EDTA plasma (n=4) | 102 | 97-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5001.9782.0121.9951.8272501.5681.6011.5851.4171251.0161.0321.0240.85662.50.5270.5180.5230.35531.20.3540.3610.3580.19015.60.2570.2480.2530.0857.80.2040.2110.2080.04000.1660.1690.168 | |||||||
货期：3-5 working days
×
