首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血小板衍生生长因子DD(PDGF-DD) 酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-DD含量。PDGF - DD是一种重要的靶点。它属血小板衍生生长因子家族，在细胞增殖、迁移与血管生成等过程起关键作用。研究机制方面，主要聚焦其与受体结合后的信号传导通路，以及在肿瘤、纤维化等疾病中的异常激活，为相关疾病治疗提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于小鼠模型研究中PDGF-DD的动态监测，如探究其在组织修复、纤维化进程或肿瘤微环境中的调控作用，支持心血管疾病、肿瘤生物学及再生医学等领域的科研实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-DD
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-96 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 84-93 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 95-104 | ||||||
回收率：
| The recovery of mouse PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 83-89 | |||||
| EDTA plasma (n=4) | 97 | 93-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.5482.6012.5752.44852.1152.0122.0641.9372.51.3571.3031.3301.2031.250.7860.7320.7590.6320.6250.4590.4620.4610.3340.3120.2920.2890.2910.1640.1560.2110.2040.2080.08100.1260.1280.127 | |||||||
货期：3-5 working days
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