首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血小板衍生生长因子CC(PDGF-CC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PDGFC含量。PDGFC即血小板衍生生长因子C，是PDGF家族成员。它在胚胎发育、组织修复等多种生理过程发挥重要作用。在研究机制上，其通过与受体结合激活下游信号通路，参与细胞的增殖、迁移等活动，在癌症等疾病中也有异常表达，为相关疾病治疗带来新思路。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于心血管疾病模型、肿瘤机制研究、细胞信号通路探索等多种基础研究场景；其定量分析对研究血管相关疾病、肿瘤微环境及再生医学具有重要意义。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pdgfc ELISA Kit; Scdgf ELISA Kit; Platelet-derived growth factor C ELISA Kit; PDGF-C ELISA Kit; Fallotein ELISA Kit; Spinal cord-derived growth factor ELISA Kit; SCDGF ELISA Kit; VEGF-E) [Cleaved into: Platelet-derived growth factor C ELISA Kit; latent form ELISA Kit; PDGFC latent form); Platelet-derived growth factor C ELISA Kit; receptor-binding form ELISA Kit; PDGFC receptor-binding form)] ELISA Kit
缩写：PDGFC
Uniprot No.：Q8CI19
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 95-105 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 89-100 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 88-101 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-99 | ||||||
回收率：
| The recovery of mouse PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 98 | 90-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.4802.4112.4462.28220001.8791.8581.8691.70510001.1511.1091.1300.9665000.6360.6250.6310.4672500.3990.3890.3940.2301250.2870.2780.2830.11962.50.2150.2100.2130.04900.1660.1620.164 | |||||||
货期：3-5 working days
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