Mouse Platelet-derived growth factor subunit BB(PDGF-BB) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL017709MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠血小板衍生生长因子β(PDGFB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PDGFB含量。PDGFB 即血小板衍生生长因子 B，在细胞生长、增殖和分化等过程中扮演关键角色。其异常表达与肿瘤生长、纤维化疾病等密切相关。研究机制主要聚焦于其与受体结合后激活下游信号通路，调控细胞行为，为相关疾病治疗提供靶点。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，其动态监测可为心血管疾病、纤维化疾病及肿瘤机制研究提供重要数据支持；适用于体外实验中PDGFB的表达水平分析，例如细胞因子调控研究、疾病模型病理分析或药物干预效果评估等科研场景；可为细胞实验、动物模型及离体组织研究提供稳定可靠的定量检测工具，助力科研人员探索PDGFB在生物学过程中的分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
PDGFB
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, cell culture supernates, tissue homogenates
检测范围：
93.75 pg/mL-6000 pg/mL
灵敏度：
23.44 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGFB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	88
	Range %	83-93
1:4	Average %	92
	Range %	85-99
1:8	Average %	85
	Range %	81-90

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
6000	2.090	2.034	2.062	1.901
3000	1.308	1.227	1.267	1.106
1500	0.864	0.828	0.846	0.685
750	0.526	0.519	0.523	0.361
375	0.334	0.321	0.327	0.166
187.5	0.243	0.253	0.248	0.087
93.75	0.201	0.194	0.198	0.036
0	0.158	0.165	0.161