首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血小板衍生生长因子BB(PDGF-BB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-BB含量。PDGF-BB 是血小板衍生生长因子异构体。其背景在于它在细胞生长、增殖等生理过程有关键作用。研究机制主要围绕其与受体结合，激活下游信号通路，在肿瘤血管生成、纤维化疾病发展等病理过程有重要影响，也是药物研发热门靶点。试剂盒检测范围为0.18 ng/mL-12 ng/mL，科研人员可将其应用于小鼠模型中的炎症反应研究、血管生成机制探索或组织再生相关实验，也可用于评估药物干预或基因修饰对 PDGF-BB 表达的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-BB
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.18 ng/mL-12 ng/mL
灵敏度：0.047 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 82-88 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 97-109 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 93-105 | |||||
回收率：
| The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-101 | ||||
| EDTA plasma (n=4) | 89 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected122.3892.5012.4452.28161.4451.5291.4871.32330.9050.9840.9450.7811.50.6560.6330.6450.4810.750.3450.3490.3470.1830.370.2870.2650.2760.1120.180.2230.2180.2210.05700.1590.1690.164 | ||||||
货期：3-5 working days
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